Methods of culturing a mammalian cell

作者：HWANG CHRISTOPHER | JOHNSON TIMOTHY | WALTHER JASON | CHENG CHENG | WANG JONATHAN | SHA NIHA | PEI SEYA

申请者：GENZYME CORPORATION

发明人：HWANG CHRISTOPHER | JOHNSON TIMOTHY | WALTHER JASON | CHENG CHENG | WANG JONATHAN | SHA NIHA | PEI SEYA 年：2022,

语种：英语

公开国家：TW 国家：中国台湾

专利申请号：TW110131996 专利申请日期：2015-12-22

公开(公告)号：TW20220078** 公开日期：2022-01-01

优先权号：US201462095734 | 2014US-62095734

分类号：C12N 5/16 20060101AFI20220101BHTW 国际主分类号：C12N 5/16 20060101AFI20220101BHTW 国际分类号：C12N-5/16 | C07K-16/00 CPC分类号：C12N-005/0043 | C12N-005/005 | C12N-2500/50 | C12N-2510/02 | C07K-016/00 | C07K-2317/14 | C12N-005/00 | C12P-021/00

法律状态：PENDING

摘要： 本案提供在液體培養基中培養哺乳動物細胞的方法，所述液體培養基包含濃度為1.8g/L或濃度高於1.8g/L的poloxamer-188或者所述液體培養基中的包含的poloxamer-188濃度基於選自下組的一個或多個因素選擇：孔徑、孔類型、氣體流速、培養基中的活細胞密度和涉及細胞應激的標記物。 | Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.